abstract：:The capability of optical coherence tomography (OCT) to perform "optical biopsy" of tissues within a depth range of 1 to 2 mm with micron-scale resolution in real time makes it a promising biomedical imaging modality for dermatologic applications. Three high-speed, spectrometer-based frequency-domain OCT systems opera...

journal_title：Journal of biomedical optics

authors： Alex A,Povazay B,Hofer B,Popov S,Glittenberg C,Binder S,Drexler W

更新日期：2010-03-01 00:00:00

abstract：:Reactive oxygen species (ROS) produced by photodynamic therapy (PDT) is recorded in vivo using a chemiluminescence (CL)-based gated optical system. A novel approach is developed to utilize the fluorescence (FL) of the CL probe as an internal fluorescence to calibrate the observed CL on pharmacokinetics of the probe in...

journal_title：Journal of biomedical optics

authors： Wei Y,Xing D,Luo S,Yang L,Chen Q

更新日期：2010-03-01 00:00:00

abstract：:Blood smear analysis has remained a crucial diagnostic tool for pathologists despite the advent of automatic analyzers such as flow cytometers and impedance counters. Though these current methods have proven to be indispensible tools for physicians and researchers alike, they provide limited information on the detaile...

journal_title：Journal of biomedical optics

authors： Mir M,Ding H,Wang Z,Reedy J,Tangella K,Popescu G

更新日期：2010-03-01 00:00:00

abstract：:We experimentally implement label-free phase microscopy using wide-field digital interferometry (WFDI) techniques to retrieve quantitative volumetric data of articular chondrocyte dynamics. Using the scanless interferometric system, we visualize chondrocyte swelling and bursting induced by hypo-osmotic pressure. Recon...

journal_title：Journal of biomedical optics

authors： Shaked NT,Finan JD,Guilak F,Wax A

更新日期：2010-01-01 00:00:00

abstract：:Cellularity of the fibrous caps of coronary atheromas, manifested by the infiltration of macrophages (average size, 20 to 30 microm), is thought to weaken the structural integrity of the cap and predispose plaques to rupture. Therefore, an imaging technology capable of identifying macrophages within fibroatheroma caps...

journal_title：Journal of biomedical optics

authors： Tanaka A,Tearney GJ,Bouma BE

更新日期：2010-01-01 00:00:00

abstract：:Photodynamic therapy (PDT) is an alternative cancer treatment modality that offers localized treatment using a photosensitizer and light. However, tumor angiogenesis is a major concern following PDT-induced hypoxia as it promotes recurrence. Bevacizumab is a monoclonal antibody that targets vascular endothelial growth...

journal_title：Journal of biomedical optics

authors： Bhuvaneswari R,Thong PS,Gan YY,Soo KC,Olivo M

更新日期：2010-01-01 00:00:00

abstract：:Laser speckle contrast imaging is a technique used for imaging blood flow without scanning. Though several studies have attempted to combine spatial and temporal statistics of laser speckle images for reducing image noise as well as preserving acceptable spatiotemporal resolution, the statistical accuracy of these spa...

journal_title：Journal of biomedical optics

authors： Qiu J,Li P,Luo W,Wang J,Zhang H,Luo Q

更新日期：2010-01-01 00:00:00

abstract：:A promising method to achieve rapid convergence for image reconstruction is introduced for the continuous-wave near-infrared (NIR) diffuse optical tomography (DOT). Tomographic techniques are usually implemented off line and are time consuming to realize image reconstruction, especially for NIR DOT. Therefore, it is e...

journal_title：Journal of biomedical optics

authors： Pan MC,Pan MC

更新日期：2010-01-01 00:00:00

abstract：:Optical coherence microscopy (OCM) combines confocal microscopy and optical coherence tomography (OCT) to improve imaging depth and contrast, enabling cellular imaging in human tissues. We aim to investigate OCM for ex vivo imaging of upper and lower gastrointestinal tract tissues, to establish correlations between OC...

journal_title：Journal of biomedical optics

authors： Aguirre AD,Chen Y,Bryan B,Mashimo H,Huang Q,Connolly JL,Fujimoto JG

更新日期：2010-01-01 00:00:00

abstract：:A new, simple, high-speed, and hardware-only integration-based fluorescence-lifetime-sensing algorithm using a center-of-mass method (CMM) is proposed to implement lifetime calculations, and its signal-to-noise-ratio based on statistics theory is also deduced. Compared to the commonly used iterative least-squares meth...

journal_title：Journal of biomedical optics

authors： Li DU,Rae B,Andrews R,Arlt J,Henderson R

更新日期：2010-01-01 00:00:00

abstract：:We present for the first time in vivo full three-dimensional (3-D) photoacoustic tomography (PAT) of the distal interphalangeal joint in a human subject. Both absorbed energy density and absorption coefficient images of the joint are quantitatively obtained using our finite-element-based photoacoustic image reconstruc...

journal_title：Journal of biomedical optics

authors： Sun Y,Sobel E,Jiang H

更新日期：2009-11-01 00:00:00

abstract：:We present a novel fluorescence imaging system developed for real-time interventional imaging applications. The system implements a correction scheme that improves the accuracy of epi-illumination fluorescence images for light intensity variation in tissues. The implementation is based on the use of three cameras oper...

journal_title：Journal of biomedical optics

authors： Themelis G,Yoo JS,Soh KS,Schulz R,Ntziachristos V

更新日期：2009-11-01 00:00:00

abstract：:Optically sectioned detection of fluorescence immunoassays using a confocal microscope enables the creation of both homo- and heterogeneous planar format assays. We report a set assays requiring optically sectioned detection using a model system and analysis procedures for separating signals of a surface layer from an...

journal_title：Journal of biomedical optics

authors： Ghafari H,Zhou Y,Ali S,Hanley QS

更新日期：2009-11-01 00:00:00

abstract：:A pilot human study is conducted to evaluate the potential of using diffuse photon density wave (DPDW) methodology at near-infrared (NIR) wavelengths (685 to 830 nm) to monitor changes in tissue hemoglobin concentration in diabetic foot ulcers. Hemoglobin concentration is measured by DPDW in 12 human wounds for a peri...

journal_title：Journal of biomedical optics

authors： Papazoglou ES,Neidrauer M,Zubkov L,Weingarten MS,Pourrezaei K

更新日期：2009-11-01 00:00:00

abstract：:We report a photonic approach for selective inactivation of viruses with a near-infrared subpicosecond laser. We demonstrate that this method can selectively inactivate viral particles ranging from nonpathogenic viruses such as the M13 bacteriophage and the tobacco mosaic virus to pathogenic viruses such as the human ...

journal_title：Journal of biomedical optics

authors： Tsen KT,Tsen SW,Fu Q,Lindsay SM,Kibler K,Jacobs B,Wu TC,Karanam B,Jagu S,Roden RB,Hung CF,Sankey OF,Ramakrishna B,Kiang JG

更新日期：2009-11-01 00:00:00

abstract：:Traditional phase-resolved Doppler optical coherence tomography (DOCT) has been reported to have potential for characterizing local fluid flow within a microporous scaffold. In this work, we apply Doppler optical microangiography (DOMAG), a new imaging technique developed by combining optical microangiography (OMAG) w...

journal_title：Journal of biomedical optics

authors： Jia Y,An L,Wang RK

更新日期：2009-09-01 00:00:00

abstract：:The epithelium (EP) thickness and the standard deviation (SD) of A-mode scan intensity in the laminar propria (LP) layer are used as effective indicators for the diagnosis of oral submucous fibrosis (OSF) based on the noninvasive clinical scanning of a swept-source optical coherence tomography (OCT) system of approxim...

journal_title：Journal of biomedical optics

authors： Lee CK,Tsai MT,Lee HC,Chen HM,Chiang CP,Wang YM,Yang CC

更新日期：2009-09-01 00:00:00

abstract：:Quantitative polarized light microscopy (qPLM) is a popular tool for the investigation of birefringent architectures in biological tissues. Collagen, the most abundant protein in mammals, is such a birefringent material. Interpretation of results of qPLM in terms of collagen network architecture and anisotropy is chal...

journal_title：Journal of biomedical optics

authors： van Turnhout MC,Kranenbarg S,van Leeuwen JL

更新日期：2009-09-01 00:00:00

abstract：:We demonstrate that using time-resolved two-photon excitation endogenous fluorescence microscopy, the cadmium (Cd)-induced cellular toxic level can be assessed by the free-to protein-bound reduced nicotinamide adenine dinucleotide (free/bound NADH) ratio in a living cell. NADH fluorescence excited at 730 nm is capture...

journal_title：Journal of biomedical optics

authors： Li D,Yang MS,Lin T,Zheng W,Qu JY

更新日期：2009-09-01 00:00:00

abstract：:Alcohol testing is an expanding area of interest due to the impacts of alcohol abuse that extend well beyond drunk driving. However, existing approaches such as blood and urine assays are hampered in some testing environments by biohazard risks. A noninvasive, in vivo spectroscopic technique offers a promising alterna...

journal_title：Journal of biomedical optics

authors： Ridder TD,Ver Steeg BJ,Laaksonen BD

更新日期：2009-09-01 00:00:00

abstract：:Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBCs) as an endogenic contrast agent. However, intermittent RBC flow in capillaries res...

journal_title：Journal of biomedical optics

authors： Yao J,Maslov K,Hu S,Wang LV

更新日期：2009-09-01 00:00:00

abstract：:Recaldent is a product of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP). The remineralizing potential of CPP-ACP per se, or when combined with 0.22% Fl gel on artificially demineralized enamel using laser florescence, is investigated. Mesial surfaces of 15 sound human molars are tested using a He-Cd lase...

journal_title：Journal of biomedical optics

authors： Elsayad I,Sakr A,Badr Y

更新日期：2009-07-01 00:00:00

abstract：:Optical microanglography (OMAG) is a recently developed imaging modality capable of volumetric imaging of dynamic blood perfusion, down to capillary level resolution, with an imaging depth up to 2.00 mm beneath the tissue surface. We report the use of OMAG to monitor the cerebral blood flow (CBF) over the cortex of mo...

journal_title：Journal of biomedical optics

authors： Jia Y,Alkayed N,Wang RK

更新日期：2009-07-01 00:00:00

abstract：:Diffusion of two model drugs-benzyl nicotinate and ibuprofen-and the plasma macromolecule albumin across atherosclerotic rabbit aorta was studied ex vivo by attenuated total reflection-Fourier transform infrared (ATR-FTIR) imaging. Solutions of these molecules were applied to the endothelial surface of histological se...

journal_title：Journal of biomedical optics

authors： Palombo F,Danoux CB,Weinberg PD,Kazarian SG

更新日期：2009-07-01 00:00:00

abstract：:Coherent anti-Stokes Raman scattering (CARS) microscopy is used to determine the distribution and concentration of selected compounds in intact human hair. By generating images based on ratiometric CARS contrast, quantitative concentration maps of both water and externally applied d-glycine are produced in the cortex ...

journal_title：Journal of biomedical optics

authors： Zimmerley M,Lin CY,Oertel DC,Marsh JM,Ward JL,Potma EO

更新日期：2009-07-01 00:00:00

abstract：:Cardiac architecture is inherently three-dimensional, yet most characterizations rely on two-dimensional histological slices or dissociated cells, which remove the native geometry of the heart. We previously developed a method for labeling intact heart sections without dissociation and imaging large volumes while pres...

journal_title：Journal of biomedical optics

authors： Huang H,Macgillivray C,Kwon HS,Lammerding J,Robbins J,Lee RT,So P

更新日期：2009-07-01 00:00:00

abstract：:We interrogate the ability of free-space fluorescence tomography to image small animals in vivo using charge-coupled device (CCD) camera measurements over 360-deg noncontact projections. We demonstrate the performance of normalized dual-wavelength measurements that are essential for in-vivo use, as they account for th...

journal_title：Journal of biomedical optics

authors： Deliolanis NC,Dunham J,Wurdinger T,Figueiredo JL,Tannous BA,Ntziachristos V

更新日期：2009-05-01 00:00:00

abstract：:In laser retinal photocoagulation, short (<20 ms) pulses have been found to reduce thermal damage to the inner retina, decrease treatment time, and minimize pain. However, the safe therapeutic window (defined as the ratio of power for producing a rupture to that of mild coagulation) decreases with shorter exposures. T...

journal_title：Journal of biomedical optics

authors： Sramek C,Paulus Y,Nomoto H,Huie P,Brown J,Palanker D

更新日期：2009-05-01 00:00:00

abstract：:In near-infrared spectroscopy, concentration changes in oxygenated and deoxygenated hemoglobin are calculated from the changes in the attenuation of the measurement light. This is done by solving a linear equation based on the modified Lambert-Beer law. To solve this equation, we need to know the partial optical pathl...

journal_title：Journal of biomedical optics

authors： Umeyama S,Yamada T

更新日期：2009-05-01 00:00:00

abstract：:In-vivo imaging of the vascular system can provide novel insight into the dynamics of vasoconstriction and vasodilation. Fourier domain optical coherence tomography (FD-OCT) is an optical, noncontact imaging technique based on interferometry of short-coherent near-infrared light with axial resolution of less than 10 m...

journal_title：Journal of biomedical optics

authors： Meissner S,Müller G,Walther J,Morawietz H,Koch E

更新日期：2009-05-01 00:00:00

abstract：:We present experiments and analyses of confocal reflectance and two-photon microscopy studies of zebra finch skull samples. The thin and hollow structure of these birds' skulls is quite translucent, which can allow in vivo transcranial two-photon imaging for brain activation monitoring. However, the skull structure is...

journal_title：Journal of biomedical optics

authors： Abi-Haidar D,Olivier T

更新日期：2009-05-01 00:00:00

abstract：:The Nikon C1 confocal laser scanning microscope is a relatively inexpensive and user-friendly instrument. We describe a straightforward method to convert the C1 for multiphoton microscopy utilizing direct coupling of a femtosecond near-infrared laser into the scan head and fiber optic transmission of emission light to...

journal_title：Journal of biomedical optics

authors： Mancuso JJ,Larson AM,Wensel TG,Saggau P

更新日期：2009-05-01 00:00:00

abstract：:The use of reduced nicotinamide adenine dinucleotide (NADH) fluorescence to gain metabolic information on kidneys in response to an alteration in oxygen availability has previously been experimentally demonstrated, but signal quantification has not, to date, been addressed. In this work the relative contribution to ra...

journal_title：Journal of biomedical optics

authors： Raman RN,Pivetti CD,Rubenchik AM,Matthews DL,Troppmann C,Demos SG

更新日期：2009-03-01 00:00:00

abstract：:Nanophotothermolysis with long laser pulses for treatment of scattered cancer cells and their clusters is introduced with the main focus on real-time monitoring of temperature dynamics inside and around individual cancer cells labeled with carbon nanotubes. This technique utilizes advanced time- and spatially-resolved...

journal_title：Journal of biomedical optics

authors： Biris AS,Boldor D,Palmer J,Monroe WT,Mahmood M,Dervishi E,Xu Y,Li Z,Galanzha EI,Zharov VP

更新日期：2009-03-01 00:00:00

abstract：:The effect of silica/gold nanoshells and titanium dioxide nanoparticles on the optical properties of skin is studied. By implementing in vivo measurements and Monte Carlo simulations, we analyze the efficiency of using these nanoparticles as contrasting agents for optical coherence tomography (OCT) imaging of skin. In...

journal_title：Journal of biomedical optics

authors： Kirillin M,Shirmanova M,Sirotkina M,Bugrova M,Khlebtsov B,Zagaynova E

更新日期：2009-03-01 00:00:00

abstract：:This study demonstrates the use of optical spectroscopy for monitoring tumor oxygenation and metabolism in response to hyperoxic gas breathing. Hemoglobin saturation and redox ratio were quantified for a set of 14 and 9 mice, respectively, measured at baseline and during carbogen breathing (95% O(2), 5% CO(2)). In par...

journal_title：Journal of biomedical optics

authors： Palmer GM,Viola RJ,Schroeder T,Yarmolenko PS,Dewhirst MW,Ramanujam N

更新日期：2009-03-01 00:00:00

abstract：:We have developed a novel parallel-plate diffuse optical tomography (DOT) system for three-dimensional in vivo imaging of human breast tumor based on large optical data sets. Images of oxy-, deoxy-, and total hemoglobin concentration as well as blood oxygen saturation and tissue scattering were reconstructed. Tumor ma...

journal_title：Journal of biomedical optics

authors： Choe R,Konecky SD,Corlu A,Lee K,Durduran T,Busch DR,Pathak S,Czerniecki BJ,Tchou J,Fraker DL,Demichele A,Chance B,Arridge SR,Schweiger M,Culver JP,Schnall MD,Putt ME,Rosen MA,Yodh AG

更新日期：2009-03-01 00:00:00

abstract：:Model-based light scattering spectroscopy (LSS) seemed a promising technique for in-vivo diagnosis of dysplasia in multiple organs. In the studies, the residual spectrum, the difference between the observed and modeled diffuse reflectance spectra, was attributed to single elastic light scattering from epithelial nucle...

journal_title：Journal of biomedical optics

authors： Lau C,Sćepanović O,Mirkovic J,McGee S,Yu CC,Fulghum S,Wallace M,Tunnell J,Bechtel K,Feld M

更新日期：2009-03-01 00:00:00

abstract：:Microstructures of unstained human melanoma skin tissues have been examined by multimodal nonlinear optical microscopy. The polarized shape of the individual melanoma cell can be readily recognized-a phenotype that has been identified in laboratory cultures as characteristic of proliferating melanocytes but has not be...

journal_title：Journal of biomedical optics

authors： Zhang K,Zhang W,Yang CY,Yang H

更新日期：2009-03-01 00:00:00

abstract：:An adaptive pulse shaper controlled by multiphoton intrapulse interference phase scanning (MIIPS) was used, together with a prism-pair, to measure and cancel high-order phase distortions introduced by a high-numerical-aperture objective and other dispersive elements of a two-photon laser-scanning microscope. The deliv...

journal_title：Journal of biomedical optics

authors： Xi P,Andegeko Y,Pestov D,Lovozoy VV,Dantus M

更新日期：2009-01-01 00:00:00